Protein having activity as an angiogenesis modulator

ABSTRACT

BTL.012 is a novel human protein useful for regulating or modulating angiogenesis. BTL.012, or variants thereof, may be employed as therapeutics in diseases such as cancer, wound healing, diabetic retinopathies, macular degeneration, and cardiovascular diseases, and other diseases or clinical conditions where angiogenesis is relevant to the causation or treatment of the disease.

BACKGROUND OF THE INVENTION

[0001] 1. Field

[0002] This invention relates to newly identified polynucleotides, polypeptides encoded by such polynucleotides, the use of such polynucleotides and polypeptides, as well as the production of such polynucleotides and polypeptides. More particularly, the polypeptide of the present invention, hereinafter referred to as BTL.012, has been identified as having a thrombospondin repeat domain and as being active in modulating angiogenesis activity.

[0003] 2. Background

[0004] The thrombospondin family of proteins includes large, multidomain glycoproteins involved in the regulation of proliferation, adhesion, migration and angiogenesis (Mosher, Ann. Rev. Med. (1990) 41: 85-97; Frazier, Curr. Opin. Cell Biol. (1990) 3: 797-99; Bornstein FASEB J. (1992) 6: 3290-99; Lahav, Biochim. Biophys. Acta (1993) 1182: 1-14). The prototype of this family is Thrombospondin-1 (TSP-1), which was first identified as a protein associated with the surfaces of thrombin-stimulated platelets (Baenziger et al., Proc. Natl. Acad. Sci. U.S.A. (1971) 68: 245-49). TSP-1 is a homotrimer with each subunit comprising a 1152 amino acid polypeptide. The complete amino acid sequence of TSP-1 has been determined from cDNA clones isolated by various groups (e.g. Lawler et al. J. Cell Biol. (1986) 103: 1635-48; Kobayashi et al., Biochemistry (1986) 25: 8418-25; and Dixit et al., Proc. Natl. Acad. Sci. U.S.A. (1986) 83: 5449-53). TSP-1 contains a heparin-binding domain, a procollagen homology domain, and three types of repeated domains termed the type 1 (TSP or properdin), type 2 (EGF-like), and type 3 (Ca⁺⁺-binding) repeats (Bornstein, FASEB J. (1992) 6: 3290-99). Five members of the TSP family have been discovered, termed as TSP-1, TSP-2, TSP-3, TSP-4 and COMP/TSP-5 (Bornstein, J. Cell Biol. (1995) 130: 503-06). TSP-1 and TSP-2 are structurally more similar to each other than to TSP-3, TSP4, or TSP-5 (Bornstein and Sage, Meth. Enzymol. (1994) 245: 62-85). Both TSP-1 and TSP-2 are secreted as disulfide-bonded homotrimers whereas TSP-3, TSP-4, and TSP-5 are pentameric. TSP-1 and TSP-2 interact with a number of cell surface receptors, including integrin v-3 LDL-related receptor protein, and heparin sulfate proteoglycans (Chen et al., J. Biol. Chem. (1994) 269: 32226-32; and Chen et al., J. Biol. Chem. (1996) 271: 15993-99).

[0005] In vitro and in vivo assays demonstrate that TSP-1 and TSP-2 act both as angiogenesis inhibitors and as potent suppressors of malignant tumor growth (Weinstat et al., Cancer Research (1994) 54: 6504-11; Bleuel et al., Proc. Natl. Acad. Sci. U.S.A. (1999) 96: 2065-70; Streit et al., Am. J. Pathol. 155: 441-52; and Streit et al., Proc. Natl. Acad. Sci. U.S.A. (1999) 96: 14888-93). TSP-1 and TSP-2 are shown to be highly expressed in developing blood vessels, indicating potential roles in primary angiogenesis (Iruela-Arispe et al., Dev. Dyn. (1993) 197: 40-56; and Reed et al., Am. J. Pathol. (1995) 147: 1068-80). Indeed, targeted disruption of the TSP-2 gene significantly increased numbers of small- and mid-sized blood vessels in several tissues including skin (Kyriakides et al., J. Cell Biol. (1998) 140: 419-13).

[0006] The regions responsible for inhibition of angiogenesis by TSP-1 have been mapped to the procollagen domain and to the type 1 repeats (Tolsma et al., J. Cell Biol. (1993) 122: 497-511). It is suggested that the inhibition of capillary growth by TSP-1 is multifactorial and involves competition for FGF-2 binding to the endothelial cell surface, binding to heparin sulfate proteoglycans, activation of TGF-beta, and/or binding to CD-36, a receptor for TSP-1 (Vogel et al., J. Cell Biochem. (1993) 53: 74-84; Taraboletti et al., Cell Growth Diff. (1997)\8: 471-79; Schultz-Cherry et al., J. Biol. Chem. (1995) 27: 7304-10; and Dawson et al., J. Cell Biol. (1997)138: 707-17). A truncated TSP subunit was found to both inhibit the proliferation of endothelial cells and to result in increased concentrations of plasminogen activator inhibitor-1, indicating that TSP may affect the process of angiogenesis through at least two mechanisms—proliferation of cells in neovascularization and degradation of the extracellular matrix (Bagavandoss et al., Biochem. Biophys. Res. Comm. (1993) 192: 325-32).

[0007] The assays for the antiangiogenesis activity include corneal pocket assay, chorioallantoic membrane angiogenesis assay (CAM), and endothelial cell proliferation and migration assays. Various TSP family proteins, including full length expressed TSP family proteins from transfected mammalian cells, various portions of the repeat domains expressed in bacterial systems, and synthesized peptides, have been used in the above mentioned assays. A recent study has identified two regions of type 1 repeats as potent inhibitors of angiogenesis (Iruela-Arispe et al., Circulation (1999) 100: 1423-31). An N-terminal tryptophan rich domain as well as a C-terminal CSVTCG (SEQ ID NO:5) sequence have been shown to independently inhibit neovascularization. The N-terminal domain showed a stronger inhibition activity against FGF-2-driven angiogenesis, whereas the second region equally blocked the angiogenesis induced by either FGF-2 or VEGF (Iruela-Arispe et al., Circulation (1999) 100: 1423-31).

[0008] Recently, a novel brain-specific angiogenesis inhibitor (BAI-1) was identified and cloned (Nishimori et al., Oncogene (1997) 15: 2145-50). It contains 5 TSP type-1 repeats. Recombinant proteins containing these repeats inhibited in vivo neovascularization induced by bFGF in the rat cornea assay. Vascularization is decreased in pulmonary adenocarcinoma expressing BAI-1 (Hatanaka et al., Int J Mol Med. (2000) 5: 181-3). Two BAI-1 homologs have recently been cloned and named as BAI-2 and BAI-3 (Shiratsuchi et al., Cytogenet. Cell Genet. (1997) 79: 103-108). Like BAI-1, BAI-3 was absent or significantly reduced in some glioblastoma cell lines, suggesting that members of this novel gene family may play important roles in suppression of glioblastoma.

[0009] Angiogenesis, the formation of new capillaries from preexisting blood vessels, is a multistep, highly orchestrated process involving vessel sprouting, endothelial cell migration, proliferation, tube differentiation, and survival. Several lines of direct evidence now suggest that angiogenesis is essential for the growth and persistence of solid tumors and their metastases (Folkman et al. (1989) Nature 339:58-61; Hori et al. (1991) Cancer Research 51:6180-84; Kim et al. (1993) Nature 362:841-844; Millauer et al. (1996) Cancer Research 56:1615-20). To stimulate angiogenesis, tumors up regulate their production of a variety of angiogenic factors, including the fibroblast growth factors (FGF and BFGF) (Kandel et al. (1991) Cell. 66:1095-104) and vascular endothelial cell growth factor/vascular permeability factor (VEGF/VPF). However, many malignant tumors also generate inhibitors of angiogenesis, including angiostatin and thrombospondin (Chen et al. (1195) Cancer Research 55:4230-33; Good et al. (1990) Proc Natl Acad Sci U S A. 87:6624-28; O'Reilly et al. (1994) Cell 79:315-28). It is postulated that theangiogenic phenotype is the result of a net balance between these positive and negative regulators of neovascularization (Good et al. (1990), supra; O'Reilly et al. (1994), supra; Parangi et al. (1996) Proc Natl Acad Sci U S A. 93:2002-07; Rastinejad et al. (1989) Cell 56:345-55).

[0010] Several other endogenous inhibitors of angiogenesis have been identified, although not all are associated with the presence of a tumor. These include platelet factor 4 (Gupta et al. (2000) Blood 95:147-55), interferon-alpha, interferon-inducible protein 10 (Angiolilloet al. (1996) Ann. N.Y. Acad. Sci. 795:158-67; Strieter et al. (1995) J. Biol. Chem. 270:27348-57), which is induced by interleukin-12 and/or interferon-gamma, gro-beta (Cao et al. (1995) J. Exp. Med. 182:2069-77), and the 16 kDa N-terminal fragment of prolactin (Clapp et al. (1999) Invest. Ophthalmol. Vis. Sci. 40:2498-505). The only known angiogenesis inhibitor which specifically inhibits endothelial cell proliferation is angiostatin (O'Reilly et al. (1994), supra). Angiostatin is an approximately 38 kiloDalton (kDa) specific inhibitor of endothelial cell proliferation. Angiostatin is an internal fragment of plasminogen containing at least three of the five kringles of plasminogen. Angiostatin has been shown to reduce tumor weight and to inhibit metastasis in certain tumor models. (O'Reilly et al. (1994), supra).

SUMMARY OF THE INVENTION

[0011] We have now discovered a new protein, hereinafter referred to as BTL.012, which has been identified as having a thrombospondin repeat domain and as being active in modulating angiogenesis activity.

[0012] The instant invention encompasses the use of BTL.012 for regulating or modulating angiogenesis. The current invention further encompasses the use of BTL.012 for the treatment of a disease or clinical condition where angiogenesis is relevant to the causation or treatment of the disease or clinical condition, including but not limited to cancer, wound healing, diabetic retinopathies, macular degeneration, and cardiovascular diseases. The instant invention also encompasses pharmaceutical compositions containing BTL.012 and the use of the pharmaceutical compositions for the treatment of the abovementioned diseases or clinical conditions.

[0013] In accordance with one aspect of the present invention, there are provided novel mature polypeptides comprising the amino acid sequence given in SEQ ID NO:1 as well as biologically active and diagnostically or therapeutically useful fragments, analogues and derivatives thereof As an additional aspect of the present invention, there are provided antibodies to the polypeptides of the present invention, especially antibodies which bind specifically to an epitope made up of the sequence described in SEQ ID NO:1 or a sequence which shares at least a 60%, preferably at least a 70%, more preferably at least an 80%, still more preferably a 90%, or most preferably at least a 95% sequence identity over at least 20, preferably at least 30, more preferably at least 40, still more preferably at least 50, or most preferably at least 100 residues with SEQ ID NO:1.

[0014] In accordance with another aspect of the present invention, there are provided isolated nucleic acid molecules encoding the polypeptides of the present invention, including mRNAs, DNAs, cDNAs, genomic DNA, as well as antisense analogs thereof and biologically active and diagnostically or therapeutically useful fragments thereof.

[0015] In accordance with still another aspect of the present invention, there are provided processes for producing such polypeptides by recombinant techniques through the use of recombinant vectors. As a further aspect of the present invention, there are provided recombinant prokaryotic and/or eukaryotic host cells comprising a nucleic acid sequence encoding a polypeptide of the present invention.

[0016] In accordance with a further aspect of the present invention, there is provided a process for utilizing such polypeptides, or polynucleotides encoding such polypeptides, for therapeutic purposes, for example, the treatment of cancer, wound healing, diabetic retinopathies, macular degeneration, and cardiovascular diseases.

[0017] In accordance with another aspect of the present invention, there are provided nucleic acid probes comprising nucleic acid molecules of sufficient length to specifically hybridize to a polynucleotide encoding a polypeptide of the present invention.

[0018] In accordance with yet another aspect of the present invention, there are provided diagnostic assays for detecting diseases or susceptibility to diseases related to mutations in a nucleic acid sequence of the present invention and for detecting over-expression or underexpression of the polypeptides encoded by such sequences.

[0019] In accordance with another aspect of the present invention, there is provided a process involving expression of such polypeptides, or polynucleotides encoding such polypeptides, for purposes of gene therapy. As used herein, gene therapy is defined as the process of providing for the expression of nucleic acid sequences of exogenous origin in an individual for the treatment of a disease condition within that individual.

BRIEF DESCRIPTION OF THE FIGURES

[0020]FIG. 1 shows the alignment of various thrombospondin domain type I repeats in Thrombospondin 1 and 2 (TSP1 & 2), Brain-specific Angiogenesis Inhibitor 1, 2, and 3 (BAI1, 2 & 3), and BTL.012. Numbers in the right column reflect the approximate location of the type 1 repeat in the full length protein, or, for BTL.012, the location in SEQ ID NO:33.

[0021]FIG. 2 shows the thromospondin domain repeat alignment with brain-specific angiogenesis inhibitor 1. (Query: is SEQ ID NO:31; Sbjct: is SEQ ID NO:32.)

[0022]FIG. 3 illustrates the dose dependent inhibitory effect of BTL.012 containing protein supernatants on capillary-like organization of HUVEC cells in MATRIGEL. Results are expressed as percentage of control, which represents the capillary-like organization of untreated HUVEC cells in MATRIGEL.

[0023]FIG. 4 illustrates the dose dependent inhibitory effect of purified BTL.012 protein on capillary-like organization of HUVEC cells in MATRIGEL. Results are expressed as percentage of control, which represents the capillary-like organization of untreated HUVEC cells in MATRIGEL.

[0024]FIG. 5 illustrates the dose dependent inhibitory effect of purified BTL.012 protein on capillary-like organization of MLuEC cells in MATRIGEL. Results are expressed as percentage of control, which represents the capillary-like organization of untreated HUVEC cells in MATRIGEL.

SPECIFIC EMBODIMENTS Materials and Methods

[0025] In vitro MATRIGEL assay: Human umbilical cord endothelial cells (HUVEC, from ATCC, Manassas, Va.) were seeded at 3×10⁴ cells per well in HUVEC complete medium. The HUVEC complete medium contained F12K medium with 2 mM L-glutamine, 100 ug/ml Heparin, 50 ug/ml Endothelial cell growth supplement (ECGS), and 10% fetal bovine serum(FBS). Murine lung endothelial cells (MLuEC) were seeded at 5×10⁴ cells per well in a complete medium containing DMEM with 2 mM L-glutamine, 1% Pen/Strep, and 10% FBS. MATRIGEL basement membrane matrix (Becton Dickinson, Franklin Lakes, N.J.) was prepared using pre-cooled, pipettes, tips, plates and tubes during handling of the matrix. The matrix was thawed at 4° C. overnight on ice, used to coat a 24-well plate (Costar, VWR, West Chester, Pa.) at 0.3 ml/well, and then polymerized at 37° C. for 2 hours. Test samples were added in 0.5 ml of complete medium per well, and cells were added in 0.5 ml of medium per well, so the total volume of medium per well was 1.0 ml. Experiments were conducted in triplicate, with varying dilutions of test samples (from 1:10 to 1:10000) or varying protein concentration (from 100 nM to 1 fM). Cells were incubated overnight at 37° C., 5% CO₂, then fixed and stained using a DIFF-QUIK staining set (VWR, West Chester, Pa.). Plates were dipped in Fixative Solution for 5 seconds, in Solution 1 for 5 seconds, and in Solution 2 for 5 seconds, then rinsed with deionized water and allowed to dry. Plates were then examined under inverted microscope, and quantitative analysis of the capillary-like structures was performed. As used herein, the term “capillary-like structures” includes organized cells in vivo or in vitro leading up to and participating in angiogenesis which results in the cells in association with each other and forming capillaries.

[0026] The polypeptides of the present invention include polypeptides having the deduced amino acid sequence given by SEQ ID NO:1. The polypeptides of the present invention may include additional amino acid sequences appended to the N- or C-terminal of the peptides having the deduced amino acid sequence given by SEQ ID NO:1. The polypeptides of the present invention may be recombinant polypeptides, natural polypeptides, or synthetic polypeptides, preferably recombinant polypeptides. As used herein, “protein” is synonymous with “polypeptide.”

[0027] The present invention further includes a polypeptide which shares at least a 60%, preferably at least a 70%, more preferably at least an 80%, still more preferably a 90%, or most preferably at least a 95% sequence identity over at least 20, preferably at least 30, more preferably at least 40, still more preferably at least 50, or most preferably at least 100 residues with SEQ ID NO:1. (Such polypeptides may be herein referred to as “polypeptides of the present invention”.) As used herein, a “BTL.012-like protein” means a polypeptide of the present invention as referred to in this paragraph. A polypeptide of the present invention is at least 20, preferably at least 30, more preferably at least 40, still more preferably at least 50, or most preferably at least 100 residues long. The invention also contemplates polypeptides which share at least a 60%, preferably at least a 70%, more preferably at least an 80%, still more preferably a 90%, or most preferably at least a 95% sequence identity over at least 20, preferably at least 30, more preferably at least 40, still more preferably at least 50, or most preferably at least 100 residues with SEQ ID NO:33. SEQ ID NO:33 is a longer novel sequence we have discovered which includes SEQ ID NO:1 as a lesser included sequence (SEQ ID NO:1 is the same as residues 654 to 861 of SEQ ID NO:33).

[0028] Such a polypeptide as described above may be (i) one in which one or more of the amino acid residues are substituted (as compared to SEQ ID NO:1) with a conserved or non-conserved amino acid residue (preferably a conserved amino acid residue) and such substituted amino acid residue may or may not be one encoded by the genetic code, or (ii) one in which one or more of the amino acid residues includes a substituent group, or (iii) one in which the mature polypeptide is fused with another compound, such as a compound to increase the half-life of the polypeptide (for example, polyethyleneglycol), or (iv) one in which additional amino acids are fused to the mature polypeptide, such as a leader or secretory sequence or a sequence which is employed for purification of the mature polypeptide or a proprotein sequence or mature protein sequence beyond the thrombospondin-repeat domain, or (v) one in which one or more amino acids are deleted from or inserted into the sequence of the polypeptide. Combinations of the above-described types of variations in the peptide sequence are within the scope of the invention. Such polypeptides are deemed to be within the scope of those skilled in the art from the teachings herein.

[0029] A polypeptide of the present invention may contain amino acids other than the 20 gene-encoded amino acids. The polypeptides may be modified by either natural processes, such as posttranslational processing, or by chemical modification techniques which are well known in the art. Such modifications are well described in basic texts and in more detailed monographs, as well as in a voluminous research literature. Modifications can occur anywhere in a polypeptide, including the peptide backbone, the amino acid side-chains, and the amino or carboxyl termini. It will be appreciated that the same type of modification may be present in the same or varying degrees at several sites in a given polypeptide. Also, a given polypeptide may contain many types of modifications. Polypeptides may be branched, for example, as a result of ubiquitination, and they may be cyclic, with or without branching. Cyclic, branched, and branched cyclic polypeptides may result from posttranslational natural processes or may be made by synthetic methods. Modifications include acetylation, acylation, ADP-ribosylation, amidation, covalent attachment of flavin, covalent attachment of a heme moiety, covalent attachment of a nucleotide or nucleotide derivative, covalent attachment of a lipid or lipid derivative, covalent attachment of phosphatidylinositol, cross-linking, cyclization, disulfide bond formation, demethylation, formation of covalent cross-links, formation of cysteine, formation of pyroglutamate, formylation, gamma-carboxylation, glycosylation, GPI anchor formation, hydroxylation, iodination, methylation, myristoylation, oxidation, pegylation, proteolytic processing, phosphorylation, prenylation, racemization, selenoylation, sulfation, transfer-RNA mediated addition of amino acids to proteins such as arginylation, and ubiquitination.

[0030] The polypeptides and polynucleotides of the present invention are preferably provided in an isolated form, and preferably are purified to homogeneity. The term “isolated” means that the material is removed from its original environment (e.g., the natural environment if it is naturally occurring). For example, a naturally occurring polynucleotide or polypeptide present in a living animal is not isolated, but the same polynucleotide or DNA or polypeptide, separated from some or all of the coexisting materials in the natural system, is isolated. Such polynucleotide could be part of a vector and/or such polynucleotide or polypeptide could be part of a composition, and still be isolated in that such vector or composition is not part of its natural environment.

[0031] As known in the art “similarity” between two polypeptides is determined by comparing the amino acid sequence and its conserved amino acid substitutes of one polypeptide to the sequence of a second polypeptide. Such conservative substitutions include those described by Dayhoff, The Atlas of Protein Sequence and Structure 5 (1978) and by Argos, EMBO J. 8: 779-785 (1989). For example, amino acids belonging to one of the following groups represent conservative changes:

[0032] ala, pro, gly, gin, asn, ser, thr;

[0033] cys, ser, tyr, thr;

[0034] val, ile, leu, met, ala, phe;

[0035] lys, arg, his;

[0036] phe, tyr, trp, his; and

[0037] asp, glu.

[0038] (Note that these grouping are examples; other groupings may represent more relevant choices.)

[0039] “Similarity” or “identity” refers to sequence conservation, or “homology”, between two or more peptides or two or more nucleic acid molecules, normally expressed in terms of percentages. When a position in the compared sequences is occupied by the same base or amino acid (“residue”), then the molecules are identical at that position. When a position in two compared peptide sequences is occupied by an amino acid with similar physical properties (a conservative substitution as determined by a given scoring matrix; similarity is thus dependent on the scoring matrix chosen), then the molecules are similar at that position. The percent identity or similarity can be maximized by aligning the compared sequences alongside each other, sliding them back and forth, and conservatively introducing gaps in the sequences where necessary. The percent identity is calculated by counting the number of identical aligning residues dividing by the total length of the aligned region, including gaps in both sequences, and multiplying by 100. Identity would thus be expressed as, e.g., “60% identity over 200 amino acids,” or “57% identity over 250 amino acids.” Similarity is calculated by counting both identities and similarities in the above calculation. For example, the alignment below has 37.5% sequence identity over 56 amino acids ((21 identities/56 residues)×100%), where 56 is the total length of the aligned region. RTPSDKPVAH--VANPQLQWLNRRANALLANGVE-RDNQLVV--EGLYLIYSQVLF 56 resid. | |  |  |   ||   | | |      |  ||   |  ||    ||| |  |  |21 ident. RAPFKKSWAYLQVAKHKLSW-NK--DGIL-HGVRYQDGNLVIQFPGLYFIICQLQF 56 resid. First sequence is SEQ ID NO:2; second sequence is SEQ ID NO:3

[0040] As a further example, the same alignment below has 55.4% sequence similarity over 56 amino acids ((31 similarities/56 residues)×100% ), where 56 is the total length of the aligned region. In this example, conservative substitutions are indicated by a plus sign and the total similarities is given by the sum of the identities and the conservative substitutions. (As noted above, determination of conservative substitutions is dependent on the scoring matrix chosen. The same alignment below may yield a different value for percent similarity using a different scoring matrix.) RTPSDKPVAH--VANPQLQWLNRRANALLANGVE-RDNQLVVE--GLYLIYSQVLF 56 resid. R P  K  A+  VA  +L W N+  + +L +GV  +D  LV++  GLY I  Q+ F 31 simil. RAPFKKSWAYLQVAKHKLSW-NK--DGIL-HGVRYQDGNLVIQFPGLYFIICQLQF 56 resid. First sequence is SEQ ID NO:2; second sequence is SEQ ID NO:3

[0041] Both of the sequences in the aligned region may be contained within longer, possibliy less homologous sequences. “Unrelated” or “non-homologous” sequences typically share less than 40% identity at the peptide level, preferably less than 25% identity.

[0042] The invention further encompasses polynucleotides which code for the above-described polypeptides of the present invention. These polynucleotides may be in the form of RNA or in the form of DNA, which DNA includes cDNA, genomic DNA, and synthetic DNA. The DNA may be double-stranded or single-stranded. The polynucleotides may include: only the coding sequence for the mature polypeptide; the coding sequence for the mature polypeptide and additional coding sequence such as a leader or secretory sequence or a proprotein sequence; the coding sequence for the mature polypeptide (and, optionally, additional coding sequence) and non-coding sequence, such as introns or non-coding -sequence 5′ and/or 3′ of the coding sequence for the mature polypeptide. Thus, the term “polynucleotide encoding a polypeptide” encompasses a polynucleotide which includes only coding sequence for the polypeptide as well as a polynucleotide which includes additional coding and/or non-coding sequence.

[0043] The present invention further relates to variants of the herein above-described polynucleotides. The variants of the polynucleotides may be naturally occurring allelic variants of the polynucleotides or non-naturally occurring variants of the polynucleotides. As known in the art, an allelic variant is an alternate form of a polynucleotide sequence which may have a substitution, deletion, or addition of one or more nucleotides which does not substantially alter the function of the encoded polypeptides. Thus, the present invention includes polynucleotides encoding the same mature polypeptide as described in Example 1, below, as well as variants of such polynucleotides which variants include deletion variants, substitution variants, and addition or insertion-variants.

[0044] The present invention also includes polynucleotides wherein the coding sequence for the mature polypeptides may be fused to a polynucleotide sequence which aids in expression and secretion of a polypeptide from a host cell, for example, a leader sequence which functions as a secretory sequence for controlling transport of a polypeptide from the cell. The polypeptide having a leader sequence is a preprotein and may have the leader sequence cleaved by the host cell to form the mature form of the polypeptide. The polynucleotides may also encode for a proprotein which is the mature protein plus additional 5′amino acid residues. A mature protein having a prosequence is a proprotein and is an inactive form of the protein. Once the prosequence is cleaved an active mature protein remains. For example, the polynucleotides of the present invention may code for a mature protein or for a protein having a prosequence or for a protein having both a prosequence and a presequence (leader sequence).

[0045] The polynucleotides of the present invention may also have the coding sequence fused in frame to a marker sequence which allows for purification of the polypeptide of the present invention. The marker sequence may be, for example, a hexa-histidine tag supplied by a pQE-9 vector to provide for purification of the mature polypeptide fused to the marker in the case of a bacterial host, or, for example, the marker sequence may be a hemagglutinin (HA) tag when a mammalian host, e.g. COS-7 cells, is used. The HA tag corresponds to an epitope derived from the influenza hemagglutinin protein. Wilson et al., 1984, Cell 37: 767. Other tag systems are well-known in the art, including the FLAG tag. The FLAG tag is based on the FLAG marker octapeptide (N-AspTyrLysAspAspAspAspLys-C) (SEQ ID NO:4). The FLAG sequence is hydrophilic and the last 5 amino acids (AspAspAspAspLys) (subsequence of SEQ ID NO:4) represent the target sequence of the protease enterokinase.

[0046] The term “gene” means the segment of DNA involved in producing a polypeptide chain; it includes regions preceding and following the coding region (leader and trailer) as well as intervening sequences (introns) between individual coding segments (exons). Fragments of the full length BTL.012 gene may be used as a hybridization probe for a cDNA library to isolate the full length gene and to isolate other genes which have a high sequence similarity to the gene or similar biological activity. Probes of this type typically have at least 20 bases and preferably have at least 30 bases and may contain, for example, 50 or more bases. The probe may also be used to identify a cDNA clone corresponding to a full length transcript and a genomic clone or clones that contain the complete BTL.012 gene including regulatory and promotor regions, exons, and introns. An example of a screen comprises isolating the coding region of the BTL.012 gene by using the known DNA sequence to synthesize an oligonucleotide probe. Labeled oligonucleotides having a sequence complementary to that of the gene of the present invention are used to screen a library of human cDNA, genomic DNA or mRNA to determine which members of the library the pr6be hybridizes to.

[0047] The present invention is directed to polynucleotides having at least a 70% identity, preferably at least 80% identity, more preferably at least a 90% identity, still more preferably at least a 95% identity, and most preferably at least 98% identity to a polynucleotide which encodes a polypeptide of the present invention, as well as fragments thereof, which fragments have at least 20 bases and preferably have at least 30 bases and more preferably have at least 50 bases, and to polypeptides encoded by such polynucleotides. One embodiment of the present invention is given by SEQ ID NO:34.

[0048] The present invention also relates to vectors that include polynucleotides of the present invention as above described, host cells that are genetically engineered with vectors of the invention, and the production of polypeptides of the invention by recombinant techniques. Host cells may be genetically engineered (transduced or transformed or transfected) with the vectors of this invention which may be, for example, a cloning vector or an expression vector. The vector may be, for example, in the form of a plasmid, a viral particle, a phage, etc. The engineered host cells can be cultured in conventional nutrient media modified as appropriate for activating promoters, selecting transformants or amplifying the BTL.012 genes. The culture conditions, such as temperature, pH and the like, are those previously used with the host cell selected for expression, and will be apparent to the ordinarily skilled artisan. The polynucleotide of the present invention may be employed for producing a polypeptide by recombinant techniques.

[0049] Thus, for example, the polynucleotide sequence may be included in any one of a variety of expression vehicles, in particular vectors or plasmids for expressing a polypeptide. Such vectors include chromosomal, non-chromosomal and synthetic DNA sequences, e.g., derivatives of SV40; bacterial plasmids; phage DNA; yeast plasmids; vectors derived from combinations of plasmids and phage DNA, viral DNA such as vaccinia, adenovirus, fowl pox virus, and pseudorabies. However, any other vector or plasmid may be used as long as they are replicable and viable in the host.

[0050] The appropriate DNA sequence may be inserted into the vector by a variety of procedures. Such procedures and others are deemed to be within the scope of those skilled in the art. The DNA sequence in the expression vector is operatively linked to an appropriate expression control sequence(s) (promoter) to direct mRNA synthesis. As representative examples of such promoters, there may be mentioned: LTR or SV40 promoter, the E. coil. lac or trp, the phage lambda PL promoter and other promoters known to control expression of genes in prokaryotic or eukaryotic cells or their viruses. The expression vector may also contain a ribosome binding site for translation initiation and a transcription terminator. The vector may also include appropriate sequences for amplifying expression. In addition, the expression vectors preferably contain a gene to provide a phenotypic trait for selection of transformed host cells such as dihydrofolate reductase or neomycin resistance for eukaryotic cell culture, or such as tetracycline or ampicillin resistance in E. coli. The vector containing the appropriate DNA sequence as herein above described, as well as an appropriate promoter or control sequence, may be employed to transform an appropriate host to permit the host to express the protein. As representative examples of appropriate hosts, there may be mentioned: bacterial cells, such as E. coli, Salmonella typhimurium, Streptomyces; fungal cells, such as yeast; insect cells, such as Drosophila S2 and Spodoptera Sf9; animal cells such as CHO, COS or Bowes melanoma; plant cells, etc. The selection of an appropriate host is deemed to be within the scope of those skilled in the art from the teachings herein.

[0051] The present invention also includes recombinant constructs comprising one or more of the sequences as broadly described above. The constructs comprise a vector, such as a plasmid or viral vector, into which a sequence of the invention has been inserted, in a forward or reverse orientation. In a preferred aspect of this embodiment, the construct further comprises regulatory sequences, including, for example, a promoter, operably linked to the sequence. Large numbers of suitable vectors and promoters are known to those of skill in the art and are commercially available. The following vectors are provided by way of example. Bacterial: pQE70, pQE60, pQE-9 (Qiagen), pBS, phagescript, psiX174, pBluescript SK, pBsKS, pNH8a, pNH16a, pNH18a, pNH46a (Stratagene), pTRC99A, pKK223-3, pKK233-3, pDR540, PRIT5 (Pharmacia). Eukaryotic: pWLneo, pSV2cat, pOG44, pXT1, pSG (Stratagene) pSVK3, pBPV, pMSG, PSVL (Pharmacia). However, any other plasmid or vector may be used as long as they are viable or can be made viable in the host. Promoter regions can be selected from any desired gene using CAT (chloramphenicol acetyl transferase) vectors or other vectors with selectable markers. Two appropriate vectors are pKK232-8 and pCM7. Particular named bacterial promoters include laci, lacZ, T3, T7, gpt, lambda PR, PL and trp. Eukarvotic promoters include CMV immediate early, HSV thymidine kinase, early and late SV40, LTRs from retrovirus, and mouse metallothionein-I. Selection of the appropriate vector and promoter is well within the level of ordinary skill in the art.

[0052] The present invention also relates to host cells containing the above-described construct. The host cell can be a higher eukaryotic cell, such as a mammalian cell, or a lower eukaryotic cell, such as a yeast cell, or the host cell can be a prokaryotic cell, such as a bacterial cell. Introduction of the construct into the host cell can be effected by calcium phosphate transfection, DEAE-Dextran mediated transfection, or electroporation. The constructs in host cells can be used in a conventional manner to produce the gene product encoded by the recombinant sequence. Alternatively, the polypeptides of the invention can be synthetically produced by conventional peptide synthesizers.

[0053] Mature proteins can be expressed in mammalian cells, yeast, bacteria, or other cells under the control of appropriate promoters. Cell-free translation systems can also be employed to produce such proteins using RNAs derived from the DNA constructs of the present invention. Appropriate cloning and expression vectors for use with prokaryotic and eukaryotic hosts are described by Sambrook et al. (Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor, N.Y., 1989; the disclosure of which is hereby incorporated by reference).

[0054] Transcription of a DNA encoding the polypeptides of the present invention by higher eukaryotes is increased by inserting an enhancer sequence into the vector. Enhancers are cis-acting elements of DNA, usually from about 10 to 300 bp, that act on a promoter to increase its transcription. Examples include the SV40 enhancer on the late side of the replication origin (bp 100 to 270), a cytomegalovirus early promoter enhancer, a polyoma enhancer on the late side of the replication origin, and adenovirus enhancers. Generally, recombinant expression vectors will include origins of replication and selectable markers permitting transformation of the host cell, e.g., the ampicillin resistance gene of E. coli and S. cerevisiae TRP1 gene, and a promoter derived from a highly-expressed gene to direct transcription of a downstream structural sequence. Such promoters can be derived from operons encoding glycolytic enzymes such as 3-phosphoglycerate kinase (PGK), alpha factor, acid phosphatase, or heat shock proteins, among others. The heterologous structural sequence is assembled in appropriate phase with translation, initiation and termination sequences, and preferably, a leader sequence capable of directing secretion of translated protein into the periplasmic space or extracellular medium. Optionally, the heterologous sequence can encode a fusion protein including an N-terminal identification peptide imparting desired characteristics, e.g., stabilization or simplified purification of expressed recombinant product.

[0055] Useful expression vectors for bacterial use are constructed by inserting a structural DNA sequence encoding a desired protein together with suitable translation, initiation, and termination signals in operable reading phase with a functional promoter. The vector will comprise one or more phenotypic selectable markers and an origin of replication to ensure maintenance of the vector and, if desirable, to provide amplification within the host. Suitable prokaryotic hosts for transformation include E. coli, Bacillus subtilis, Salmonella typhimurium and various species within the genera Pseudomonas, Streptomyces, and Staphylococcus, although others may also be employed as a matter of choice. Useful expression vectors for bacterial use can comprise a selectable marker and bacterial origin of replication derived from commercially available plasmids comprising genetic elements of the well known cloning vector pBR322 (ATCC 37017). Such commercial vectors include, for example, pKK223-3 (Pharmacia Fine Chemicals, Uppsala, Sweden) and GEM1 (Promega Biotec, Madison, Wis.) These pBR322 “backbone” sections are combined with an appropriate promoter and the structural sequence to be expressed.

[0056] After transformation of a suitable host strain and growth of the host strain to an appropriate cell density, the selected promoter may be de-repressed, if necessary, by appropriate means (e.g., temperature shift or chemical induction) and the cells may be cultured for an additional period. Cells are typically harvested by centrifugation, disrupted by physical or chemical means, and the resulting crude extract retained for further purification. Microbial cells employed in expression of proteins can be disrupted by any convenient method, including freeze-thaw cycling, sonication, mechanical disruption, or use of cell lysing agents.

[0057] Various mammalian cell culture systems can also be employed to express recombinant protein. Examples of mammalian expression systems include the COS-7 lines of monkey kidney fibroblasts (82) and other cell lines capable of expressing protein from a compatible vector, for example, the C127, 3T3, CHO, HeLa and BHK cell lines. Mammalian expression vectors will generally comprise an origin of replication, a suitable promoter and enhancer, and also any necessary ribosome binding sites, polyadenylation site, splice donor and acceptor sites, transcription termination sequences, and 5′ flanking nontranscribed sequences. DNA sequences derived from the SV40 viral genome, for example, SV40 origin, early promoter, enhancer, splice, and polyadenylation sites may be used to provide the required non-transcribed genetic elements.

[0058] The polypeptide of the present invention may be recovered and purified from recombinant cell cultures by methods used heretofore, including ammonium sulfate or ethanol precipitation, acid extraction, anion or cation exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxyapatite chromatography and lectin chromatography. Protein refolding steps can be used, as necessary, in completing configuration of the mature protein. Finally, high performance liquid chromatography (HPLC) can be employed for final purification steps.

[0059] The polypeptide of the present invention may be a naturally purified product, or a product of chemical synthetic-procedures, or produced by recombinant techniques from a prokaryotic or eukaryotic host (for example, by bacterial, yeast, higher plant, insect and mammalian cells in culture). Depending upon the host employed in a recombinant production procedure, the polypeptides of the present invention may be glycosylated with mammalian or other eukaryotic carbohydrates or may be non-glycosylated. Polypeptides of the present invention may also include an initial methionine amino acid residue.

[0060] Polypeptides of the present invention, or polynucleotides coding for polypeptides of the present invention, may be used in a process of gene therapy. Such gene therapy may be involved in the treatment of a disease or clinical condition which may include but not limited to cancer, wound healing, diabetic retinopathies, macular degeneration, and cardiovascular diseases. Fr example, cells may be engineered with a polynucleotide (DNA or RNA) encoding for the polypeptide ex vivo, the engineered cells are then provided to a patient to be treated with the polypeptide. Such methods are well-known in the art. For example, cells may be engineered by procedures known in the art by use of a retroviral particle containing RNA encoding for the polypeptide of the present invention.

[0061] Both in vitro and in vivo gene therapy methodologies are contemplated. Several methods for transferring potentially therapeutic genes to defined cell populations are known. See, e.g., Mulligan (1993) Science 260: 926-31. These methods include:

[0062] 1) Direct gene transfer. See, e.g., Wolff et al (1990) Science 247:1465-68.

[0063] 2) Liposome-mediated DNA transfer. See, e.g., Caplen at al. (1995) Nature Med. 3: 39-46; Crystal (1995) Nature Med. 1:15-17; Gao and Huang (1991) Biochem. Biophys. Res. Comm. 179:280-85.

[0064] 3) Retrovirus-mediated DNA transfer. See, e.g., Kay et al. (1993) Science, 262:117-19; Anderson (1992) Science 256:808-13. Retroviruses from which the retroviral plasmid vectors hereinabove mentioned may be derived include, but are not limited to, Moloney Murine Leukemia Virus, spleen necrosis virus, retroviruses such as Rous Sarcoma Virus, Harvey Sarcoma Virus, avian leukosis virus, gibbon ape leukemia virus, human immunodeficiency virus, adenovirus, Myeloproliferative Sarcoma Virus, and mammary tumor virus. In one embodiment, the retroviral plasmid vector is derived from Moloney Murine Leukemia Virus.

[0065] 4) DNA Virus-mediated DNA transfer. Such DNA viruses include adenoviruses (preferably Ad-2 or Ad-5 based vectors), herpes viruses (preferably herpes simplex virus based vectors), and parvoviruses (preferably “defective” or non-autonomous parvovirus based vectors, more preferably adeno-associated virus based vectors, most preferably AAV-2 based vectors). See, e.g., Ali et al. (1994) Gene Therapy, 1:367-84; U.S. Pat. No. 4,797,368, incorporated herein by reference, and U.S. Pat. No. 5,139,941, incorporated herein by reference. Adenoviruses have the advantage that they have a broad host range, can infect quiescent or terminally differentiated cells, such as neurons or hepatocytes, and appear essentially non-oncogenic. Adenoviruses do not appear to integrate into the host genome. Because they exist extrachromosomally, the risk of insertional mutagenesis is greatly reduced. Adeno-associated viruses exhibit similar advantages as adenoviral-based vectors. However, AAVs exhibit site-specific integration on human chromosome 19.

[0066] The choice of a particular vector system for transferring the gene of interest will depend on a variety of factors. One important factor is the nature of the target cell population. Although retroviral vectors have been extensively studied and used in a number of gene therapy applications, these vectors are generally unsuited for infecting non-dividing cells. In addition, retroviruses have the potential for oncogenicity. However, recent developments in the field of lentiviral vectors may circumvent some of these limitations. See Naldini et al. (1996) Science 272:263-7.

[0067] According to this embodiment, gene therapy with DNA encoding a polypeptide of the present invention is provided to a patient in need thereof, concurrent with, or immediately after diagnosis. The skilled artisan will appreciate that any suitable gene therapy vector containing DNA encoding a polypeptide of the present invention may be used in accordance with this embodiment. The techniques for constructing such a vector are known. See, e.g., Anderson (1998) Nature, 392 25-30; Verma (1998) Nature, 389 239-42. Introduction of the vector to the target site may be accomplished using known techniques.

[0068] The present invention also relates to a diagnostic assay for detecting levels of polypeptides of the present invention, e.g. in various tissues, since an over-expression of the proteins compared to normal control tissue samples may detect the presence of abnormal cellular proliferation, for example, a tumor. Assays used to detect levels of protein in a sample derived from a host are well-known to those of skill in the art and include radioimmunoassays, competitive-binding assays, Western Blot analysis, ELISA assays and “sandwich” type assays.

[0069] The polypeptides of the present invention can be used as an immunogen to produce antibodies thereto. These antibodies can be, for example, polyclonal or monoclonal antibodies. The present invention also includes chimeric, single chain, and humanized antibodies, as well as Fab fragments, or the product of an Fab expression library. Various procedures known in the art may be used for the production of such antibodies and fragments.

[0070] Antibodies generated against the polypeptides of the present invention can be obtained by direct injection of the polypeptides into an animal or by administering the polypeptides to an animal, preferably a nonhuman. The antibody so obtained will then bind the polypeptides itself In this manner, even a sequence encoding only a fragment of the polypeptides can be used to generate antibodies binding the whole native polypeptides. Such antibodies can then be used to isolate the polypeptide from tissue expressing that polypeptide or as a diagnostic reagent.

[0071] For preparation of monoclonal antibodies, any technique which provides antibodies produced by continuous cell line cultures can be used. See generally Antibodies: A Laboratory Manual, Harlow and Lane, eds. (1988) Cold Spring Harbor Laboratory. Examples include the hybridoma technique (Kohler and Milstein (1975) Nature 256:495-97), the trioma technique, the human B-cell hybridoma technique (Kozbor et al. (1983) Immunology Today, 4:72), and the EBV-hybridoma technique to produce human monoclonal antibodies (Cole et al. (1985) in Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, Inc., pp. 77-96).

[0072] Techniques described for the production of single chain antibodies (U.S. Pat. No. 4,946,778) can be adapted to produce single chain antibodies to immunogenic polypeptide products of this invention. Also, transgenic mice may be used to express humanized antibodies to immunogenic polypeptide products of this invention. Humanized antibodies may also be produced by methods described in U.S. Pat. Nos. 5,530,101; 5,585,089; 5,693,761; and 5,693,762, incorporated herein by reference.

Identification of Novel Protein and Comparison with Known Sequences

[0073] Random 5′ and 3′ sequences were obtained from a cDNA library of clones constructed from poly-A+ RNA prepared from mesenchymal stem cells (MSC) treated with dexamethasone. All these sequences were searched against the Genbank, Genpept, mm_uni_all, rn_uni_all, gbest, and hs_uni_fl databases using Blastn and Blastx (Altschul et al., Basic Local Alignment Search Tool, J. Mol. Biol. (1990) 215: 403-10). These database files are publicly available at the NCBI on-line database at http://www.ncbi.nih.nlm.gov (National Cener for Biotechnology Information, Bethesada, Md.). The _uni_database files refer to the Unigene files at NCBI for mm (mouse) rn (rat) and hs (human). One sequence, denoted M2DEX19_A5.T7X, was discovered to be homologous to a predicted Ig superfamily repeat (I-type). The M2DEX19_A5.T7X sequence was then used to screen a human fetal tissue cDNA libarary. One positive clone was identified from this library and sequenced using the ABI dye terminator method. The final sequence was aligned using the Sequencher program (Gene Codes Corp., Ann Arbor, Mich.). The resulting sequence contained a 2.4 kb segment with an open reading frame encoding a peptide of 796 amino acids. The amino acid sequence was analyzed by Profile scan algorithm (Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland; software by Phillip Bucher, available at: http://www.isrec.isb-sib.ch/sib-isrec/pftools). Six thrombospondin type I repeats are located at the C-terminus of this peptide (FIG. 1).

[0074] A BLASTX search of this thrombospondin repeat domain showed that it shares 59% homology and 46% identity with human brain-specific angiogenesis inhibitor I (see FIG. 2). A recent search against available public databases, including Genbank and Genpept, confirmed that this sequence was a novel sequence.

[0075] A portion (sequence given by SEQ ID NO:1) of the thrombospondin repeat domain was cloned into pFLAG-CMV-1 vector. The pFLAG-CMV-1 vector is a transient expression vector for expression and secretion of N-terminal FLAG fusion proteins in mammalian cells. The preprotrypsin leader sequence precedes the FLAG sequence. Transcription of FLAG-fusion constructs is driven by the promoter-regulatory region of the human cytomegalovirus. A fusion protein, FLAG-BTL012, was expressed with a preprotrypsin signal peptide at its N-terminus followed by a FLAG tag. The signal peptide and the FLAG tag are not expected to adversely interact with the remaining protein structure, thus allowing the fusion protein to be an effective model for the BTL.012 protein which lacks the signal peptide/FLAG tag and other BTL.012-like proteins, including the protein described by SEQ ID NO:33. The pFLAG-CMV-1 vector containing the BTL.012 coding sequence was then transfected into HEK293EBNA cells (Invitrogen, Carlsbad, Calif.). Three days after transfection, the supernatant and the pellet were collected. Western blot analysis of the supernatant and pellet revealed that this fusion protein was expressed in HEK293EBNA cells and secreted into the conditioned medium.

EXAMPLE 1

[0076] The effect of BTL.012 protein supernatants was evaluated in vitro using the HUVEC MATRIGEL assay. This assay mimics endothelial cell capillary organization and is a standard in vitro assay used to evaluate angiogenic mechanisms.

[0077] Conditioned medium was collected from HEK293EBNA cells producing the FLAG-BTLO12 fusion protein as above. The conditioned medium was centrifuged to remove cell debris and the supernatants were recovered. The supernatants were added at different concentrations to HUVEC grown in culture on MATRIGEL: Twenty-four hours later cells were fixed and evaluated for capillary-like organization. Measurement of the capillary-like structures in each well allows a quantitative analysis of the biological effect of tested compounds.

[0078] The BTL.0 1 2-containing supernatants significantly inhibited HUVEC capillary-like organization. Results from a series of representative experiments are presented in FIG. 3. In this experiment supernatants containing BTL.012 protein were added to the cells at various dilutions from 1:10 to 1:10,000 with log increment. Four different clones labeled BTL012/1, BTL012/2, BTL012/3 and BTL012/4 were used to generate BTL.012-protein-containing supernatants. The results show that the supernatants inhibited capillary-like organization in a dose dependent manner (see FIG. 3). In this experiment, IL-8-TVR, an IL-8 mutein that has been shown to have an inhibitory effect in this assay, was added at a concentration of 250 nM. As a negative control, protein supernatant from cells that have been transfected with an empty vector containing the CMV-1 promoter alone (pFLAG-CMV-1) was added to the cells at the highest dilution used with the test protein supernatants. This control did not have any significant effect on HUVEC capillary-like organization.

EXAMPLE 2

[0079] The effect of BTL.012 purified protein was evaluated in vitro using the HUVEC and MLuEC cells in the MATRIGEL assay. Supernatants were collected from HEK293EBNA cells producing the FLAG-BTL012 fusion protein, as above. The supernatants were filtered through a 0.22 um filter before the purification. The filtered supernatant was then added to an anti-FLAG M2 affinity column prepared by covalently attaching the purified murine IgG1 M2 monoclonal antibody to agarose beads. The column was washed three times with TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.4), and BTL.012 was then eluted with 100 ug/ml FLAG peptide. The BTL.012 containing elute was then dialysed to eliminate the small FLAG peptide contamination. Test samples of the protein were added at different concentrations to HUVEC or MLuEC grown in culture on MATRIGEL. Twenty-four hours later cells were fixed and evaluated for capillary-like organization.

[0080] The BTL.012 protein significantly inhibited HUVEC or MLuEC capillary-like organization. Results are presented in FIGS. 4 and 5. In these experiments BTL.012 protein was added to the cells at various concentrations from 100 nM to 1 fM with log increment. The results show that the protein inhibited capillary-like organization in a dose dependent manner (see FIGS. 4 and 5). In these experiments, IL-8-TVR, an IL-8 mutein that has been shown to have an inhibitory effect in this assay, was added at a concentration of 250 nM. As a negative control, the diluent (D-PBS) was added to the cells at the highest volume used with the test protein. This control did not have any significant effect on the capillary-like organization.

Conclusion

[0081] This invention may be relevant to any disease where angiogenesis is involved, including but not limited to cancer, wound healing, diabetic retinopathies, macular degeneration, and cardiovascular diseases. In addition to their potential therapeutic use, the polypeptides of the present invention may find use in diagnostic applications, as may the polynucleotides which code for the polypeptides of the present invention, and as may antibodies to the polypeptides of the present invention.

[0082] The above examples are intended to illustrate the invention and it is thought variations will occur to those skilled in the art. Accordingly, it is intended that the scope of the invention should be limited only by the claims below.

1 34 1 208 PRT Homo sapiens 1 Gln Val His Gly Gly Phe Ser Gln Trp Ser Ala Trp Arg Ala Cys Ser 1 5 10 15 Val Thr Cys Gly Lys Gly Ile Gln Lys Arg Ser Arg Leu Cys Asn Gln 20 25 30 Pro Leu Pro Ala Asn Gly Gly Lys Pro Cys Gln Gly Ser Asp Leu Glu 35 40 45 Met Arg Asn Cys Gln Asn Lys Pro Cys Pro Val Asp Gly Ser Trp Ser 50 55 60 Glu Trp Ser Leu Trp Glu Glu Cys Thr Arg Ser Cys Gly Arg Gly Asn 65 70 75 80 Gln Thr Arg Thr Arg Thr Cys Asn Asn Pro Ser Val Gln His Gly Gly 85 90 95 Arg Pro Cys Glu Gly Asn Ala Val Glu Ile Ile Met Cys Asn Ile Arg 100 105 110 Pro Cys Pro Val His Gly Ala Trp Ser Ala Trp Gln Pro Trp Gly Thr 115 120 125 Cys Ser Glu Ser Cys Gly Lys Gly Thr Gln Thr Arg Ala Arg Leu Cys 130 135 140 Asn Asn Pro Pro Pro Ala Phe Gly Gly Ser Tyr Cys Asp Gly Ala Glu 145 150 155 160 Thr Gln Met Gln Val Cys Asn Glu Arg Asn Cys Pro Ile His Gly Lys 165 170 175 Trp Ala Thr Trp Ala Ser Trp Ser Ala Cys Ser Val Ser Cys Gly Gly 180 185 190 Gly Ala Arg Gln Arg Thr Arg Gly Cys Ser Asp Pro Val Pro Gln Tyr 195 200 205 2 51 PRT Artificial Sequence Random sequence 2 Arg Thr Pro Ser Asp Lys Pro Val Ala His Val Ala Asn Pro Gln Leu 1 5 10 15 Gln Trp Leu Asn Arg Arg Ala Asn Ala Leu Leu Ala Asn Gly Val Glu 20 25 30 Arg Asp Asn Gln Leu Val Val Glu Gly Leu Tyr Leu Ile Tyr Ser Gln 35 40 45 Val Leu Phe 50 3 52 PRT Artificial Sequence Random sequence 3 Arg Ala Pro Phe Lys Lys Ser Trp Ala Tyr Leu Gln Val Ala Lys His 1 5 10 15 Lys Leu Ser Trp Asn Lys Asp Gly Ile Leu His Gly Val Arg Tyr Gln 20 25 30 Asp Gly Asn Leu Val Ile Gln Phe Pro Gly Leu Tyr Phe Ile Ile Cys 35 40 45 Gln Leu Gln Phe 50 4 8 PRT Artificial Sequence FLAG sequence for expressed protein 4 Asp Tyr Lys Asp Asp Asp Asp Lys 1 5 5 6 PRT Artificial Sequence Sequence with antineoangiogenic activity 5 Cys Ser Val Thr Cys Gly 1 5 6 50 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 6 Asp Gly Trp Ser Pro Trp Ser Glu Trp Thr Ser Cys Ser Thr Ser Cys 1 5 10 15 Gly Asn Gly Ile Gln Gln Arg Gly Arg Ser Cys Asp Ser Leu Asn Asn 20 25 30 Arg Cys Glu Gly Ser Ser Val Gln Thr Arg Thr Cys His Ile Gln Glu 35 40 45 Cys Asp 50 7 55 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 7 Gly Gly Trp Ser His Trp Ser Pro Trp Ser Ser Cys Ser Val Thr Cys 1 5 10 15 Gly Asp Gly Val Ile Thr Arg Ile Arg Leu Cys Asn Ser Pro Ser Pro 20 25 30 Gln Met Asn Gly Lys Pro Cys Glu Gly Glu Ala Arg Glu Thr Lys Ala 35 40 45 Cys Lys Lys Asp Ala Cys Pro 50 55 8 55 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 8 Gly Gly Trp Gly Pro Trp Ser Pro Trp Asp Ile Cys Ser Val Thr Cys 1 5 10 15 Gly Gly Gly Val Gln Lys Arg Ser Arg Leu Cys Asn Asn Pro Thr Pro 20 25 30 Gln Phe Gly Gly Lys Asp Cys Val Gly Asp Val Thr Glu Asn Gln Ile 35 40 45 Cys Asn Lys Gln Asp Cys Pro 50 55 9 50 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 9 Glu Gly Trp Ser Pro Trp Ala Glu Trp Thr Gln Cys Ser Val Thr Cys 1 5 10 15 Gly Ser Gly Thr Gln Gln Arg Gly Arg Ser Cys Asp Val Thr Ser Asn 20 25 30 Thr Cys Leu Gly Pro Ser Ile Gln Thr Arg Ala Cys Ser Leu Ser Lys 35 40 45 Cys Asp 50 10 55 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 10 Gly Gly Trp Ser His Trp Ser Pro Trp Ser Ser Cys Ser Val Thr Cys 1 5 10 15 Gly Val Gly Asn Ile Thr Arg Ile Arg Leu Cys Asn Ser Pro Val Pro 20 25 30 Gln Met Gly Gly Lys Asn Cys Lys Gly Ser Gly Arg Glu Thr Lys Ala 35 40 45 Cys Gln Gly Ala Pro Cys Pro 50 55 11 55 PRT Artificial Sequence Isolated type 1 thrombospondin sequence 11 Gly Arg Trp Ser Pro Trp Ser Pro Trp Ser Ala Cys Thr Val Thr Cys 1 5 10 15 Ala Gly Gly Ile Arg Glu Arg Thr Arg Val Cys Asn Ser Pro Glu Pro 20 25 30 Gln Tyr Gly Gly Lys Ala Cys Val Gly Asp Val Gln Glu Arg Gln Met 35 40 45 Cys Asn Lys Arg Ser Cys Pro 50 55 12 54 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 12 Gly Gly Trp Lys Leu Trp Ser Leu Trp Gly Glu Cys Thr Arg Asp Cys 1 5 10 15 Gly Gly Gly Leu Gln Thr Arg Thr Arg Thr Cys Leu Pro Ala Pro Gly 20 25 30 Val Glu Gly Gly Gly Cys Glu Gly Val Leu Glu Glu Gly Arg Gln Cys 35 40 45 Asn Arg Glu Ala Cys Gly 50 13 53 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 13 Pro Ala Ala Glu Glu Trp Ser Pro Trp Ser Val Cys Ser Ser Thr Cys 1 5 10 15 Gly Glu Gly Trp Gln Thr Arg Thr Arg Phe Cys Val Ser Ser Ser Tyr 20 25 30 Ser Thr Gln Cys Ser Gly Pro Leu Arg Glu Gln Arg Leu Cys Asn Asn 35 40 45 Ser Ala Val Cys Pro 50 14 53 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 14 Gly Ala Trp Asp Glu Trp Ser Pro Trp Ser Leu Cys Ser Ser Thr Cys 1 5 10 15 Gly Arg Gly Phe Arg Asp Arg Thr Arg Thr Cys Arg Pro Pro Gln Phe 20 25 30 Gly Gly Asn Pro Cys Glu Gly Pro Glu Lys Gln Thr Lys Phe Cys Asn 35 40 45 Ile Ala Leu Cys Pro 50 15 53 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 15 Gly Asn Trp Asn Glu Trp Ser Ser Trp Ser Ala Cys Ser Ala Ser Cys 1 5 10 15 Ser Gln Gly Arg Gln Gln Arg Thr Arg Glu Cys Asn Gly Pro Ser Tyr 20 25 30 Gly Gly Ala Glu Cys Gln Gly His Trp Val Glu Thr Arg Asp Cys Phe 35 40 45 Leu Gln Gln Cys Pro 50 16 53 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 16 Gly Lys Trp Gln Ala Trp Ala Ser Trp Gly Ser Cys Ser Val Thr Cys 1 5 10 15 Gly Ala Gly Ser Gln Arg Arg Glu Arg Val Cys Ser Gly Pro Phe Phe 20 25 30 Gly Gly Ala Ala Cys Gln Gly Pro Gln Asp Glu Tyr Arg Gln Cys Gly 35 40 45 Thr Gln Arg Cys Pro 50 17 53 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 17 Pro Ala Ala Glu Glu Trp Ser Pro Trp Ser Val Cys Ser Leu Thr Cys 1 5 10 15 Gly Gln Gly Leu Gln Val Arg Thr Arg Ser Cys Val Ser Ser Pro Tyr 20 25 30 Gly Thr Leu Cys Ser Gly Pro Leu Arg Glu Thr Arg Pro Cys Asn Asn 35 40 45 Ser Ala Thr Cys Pro 50 18 53 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 18 Gly Val Trp Glu Glu Trp Gly Ser Trp Ser Leu Cys Ser Arg Ser Cys 1 5 10 15 Gly Arg Gly Ser Arg Ser Arg Met Arg Thr Cys Val Pro Pro Gln His 20 25 30 Gly Gly Lys Ala Cys Glu Gly Pro Glu Leu Gln Thr Lys Leu Cys Ser 35 40 45 Met Ala Ala Cys Pro 50 19 53 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 19 Gly Gln Trp Leu Glu Trp Gly Pro Trp Gly Pro Cys Ser Thr Ser Cys 1 5 10 15 Ala Asn Gly Thr Gln Gln Arg Ser Arg Lys Cys Ser Val Ala Gly Pro 20 25 30 Ala Trp Ala Thr Cys Thr Gly Ala Leu Thr Asp Thr Arg Glu Cys Ser 35 40 45 Asn Leu Glu Cys Pro 50 20 53 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 20 Ser Lys Trp Gly Pro Trp Asn Ala Trp Ser Leu Cys Ser Lys Thr Cys 1 5 10 15 Asp Thr Gly Trp Gln Arg Arg Phe Arg Met Cys Gln Ala Thr Gly Thr 20 25 30 Gln Gly Tyr Pro Cys Glu Gly Thr Gly Glu Glu Val Lys Pro Cys Ser 35 40 45 Glu Lys Arg Cys Pro 50 21 52 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 21 Ser Gly Val Glu Glu Trp Ser Gln Trp Ser Thr Cys Ser Val Thr Cys 1 5 10 15 Gly Gln Gly Ser Gln Val Arg Thr Arg Thr Cys Val Ser Pro Tyr Gly 20 25 30 Thr His Cys Ser Gly Pro Leu Arg Glu Ser Arg Val Cys Asn Asn Thr 35 40 45 Ala Leu Cys Pro 50 22 53 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 22 Gly Val Trp Glu Glu Trp Ser Pro Trp Ser Leu Cys Ser Phe Thr Cys 1 5 10 15 Gly Arg Gly Gln Arg Thr Arg Thr Arg Ser Cys Thr Pro Pro Gln Tyr 20 25 30 Gly Gly Arg Pro Cys Glu Gly Pro Glu Thr His His Lys Pro Cys Asn 35 40 45 Ile Ala Leu Cys Pro 50 23 53 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 23 Gly Gln Trp Gln Glu Trp Ser Ser Trp Ser Gln Cys Ser Val Thr Cys 1 5 10 15 Ser Asn Gly Thr Gln Gln Arg Ser Arg Gln Cys Thr Ala Ala Ala His 20 25 30 Gly Gly Ser Glu Cys Arg Gly Pro Trp Ala Glu Ser Arg Glu Cys Tyr 35 40 45 Asn Pro Glu Cys Thr 50 24 53 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 24 Gly Gln Trp Asn Gln Trp Gly His Trp Ser Gly Cys Ser Lys Ser Cys 1 5 10 15 Asp Gly Gly Trp Glu Arg Arg Ile Arg Thr Cys Gln Gly Ala Val Ile 20 25 30 Thr Gly Gln Gln Cys Glu Gly Thr Gly Glu Glu Val Arg Arg Cys Ser 35 40 45 Glu Gln Arg Cys Pro 50 25 55 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 25 Gly Gly Phe Ser Gln Trp Ser Ala Trp Arg Ala Cys Ser Val Thr Cys 1 5 10 15 Gly Lys Gly Ile Gln Lys Arg Ser Arg Leu Cys Asn Gln Pro Leu Pro 20 25 30 Ala Asn Gly Gly Lys Pro Cys Gln Gly Ser Asp Leu Glu Met Arg Asn 35 40 45 Cys Gln Asn Lys Pro Cys Pro 50 55 26 55 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 26 Gly Ser Trp Ser Glu Trp Ser Leu Trp Glu Glu Cys Thr Arg Ser Cys 1 5 10 15 Gly Arg Gly Asn Gln Thr Arg Thr Arg Thr Cys Asn Asn Pro Ser Val 20 25 30 Gln His Gly Gly Arg Pro Cys Glu Gly Asn Ala Val Glu Ile Ile Met 35 40 45 Cys Asn Ile Arg Pro Cys Pro 50 55 27 55 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 27 Gly Ala Trp Ser Ala Trp Gln Pro Trp Gly Thr Cys Ser Glu Ser Cys 1 5 10 15 Gly Lys Gly Thr Gln Thr Arg Ala Arg Leu Cys Asn Asn Pro Pro Pro 20 25 30 Ala Phe Gly Gly Ser Tyr Cys Asp Gly Ala Glu Thr Gln Met Gln Val 35 40 45 Cys Asn Glu Arg Asn Cys Pro 50 55 28 55 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 28 Gly Lys Trp Ala Thr Trp Ala Ser Trp Ser Ala Cys Ser Val Ser Cys 1 5 10 15 Gly Gly Gly Ala Arg Gln Arg Thr Arg Gly Cys Ser Asp Pro Val Pro 20 25 30 Gln Tyr Gly Gly Arg Lys Cys Glu Gly Ser Asp Val Gln Ser Asp Phe 35 40 45 Cys Asn Ser Asp Pro Cys Pro 50 55 29 55 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 29 Gly Asn Trp Ser Pro Trp Ser Gly Trp Gly Thr Cys Ser Arg Thr Cys 1 5 10 15 Asn Gly Gly Gln Met Arg Arg Tyr Arg Thr Cys Asp Asn Pro Pro Pro 20 25 30 Ser Asn Gly Gly Arg Ala Cys Gly Gly Pro Asp Ser Gln Ile Gln Arg 35 40 45 Cys Asn Thr Asp Met Cys Pro 50 55 30 55 PRT Artificial Sequence Isolated type 1 thrombospondin domain sequence 30 Gly Ser Trp Gly Ser Trp His Ser Trp Ser Gln Cys Ser Ala Ser Cys 1 5 10 15 Gly Gly Gly Glu Lys Thr Arg Lys Arg Leu Cys Asp His Pro Val Pro 20 25 30 Val Lys Gly Gly Arg Pro Cys Pro Gly Asp Thr Thr Gln Val Thr Arg 35 40 45 Cys Asn Val Gln Ala Cys Pro 50 55 31 197 PRT Homo sapiens 31 Gln Trp Ser Ala Trp Arg Ala Cys Ser Val Thr Cys Gly Lys Gly Ile 1 5 10 15 Gln Lys Arg Ser Arg Leu Cys Asn Gln Pro Leu Pro Ala Asn Gly Gly 20 25 30 Lys Pro Cys Gln Gly Ser Asp Leu Glu Met Arg Asn Cys Gln Asn Lys 35 40 45 Pro Cys Pro Val Asp Gly Ser Trp Ser Glu Trp Ser Leu Trp Glu Glu 50 55 60 Cys Thr Arg Ser Cys Gly Arg Gly Asn Gln Thr Arg Thr Arg Thr Cys 65 70 75 80 Asn Asn Pro Ser Val Gln His Gly Gly Arg Pro Cys Glu Gly Asn Ala 85 90 95 Val Glu Ile Ile Met Cys Asn Ile Arg Pro Cys Pro Val His Gly Ala 100 105 110 Trp Ser Ala Trp Gln Pro Trp Gly Thr Cys Ser Glu Ser Cys Gly Lys 115 120 125 Gly Thr Gln Thr Arg Ala Arg Leu Cys Asn Asn Pro Pro Pro Ala Phe 130 135 140 Gly Gly Ser Tyr Cys Asp Gly Ala Glu Thr Gln Met Gln Val Cys Asn 145 150 155 160 Glu Arg Asn Cys Pro Ile His Gly Lys Trp Ala Thr Trp Ala Ser Trp 165 170 175 Ser Ala Cys Ser Val Ser Cys Gly Gly Gly Ala Arg Gln Arg Thr Arg 180 185 190 Gly Cys Ser Asp Pro 195 32 194 PRT Homo sapiens 32 Glu Trp Ser Pro Trp Ser Val Cys Ser Ser Thr Cys Gly Glu Gly Trp 1 5 10 15 Gln Thr Arg Thr Arg Phe Cys Val Ser Ser Ser Tyr Ser Thr Gln Cys 20 25 30 Ser Gly Pro Leu Arg Glu Gln Arg Leu Cys Asn Asn Ser Ala Val Cys 35 40 45 Pro Val His Gly Ala Trp Asp Glu Trp Ser Pro Trp Ser Leu Cys Ser 50 55 60 Ser Thr Cys Gly Arg Gly Phe Arg Asp Arg Thr Arg Thr Cys Arg Pro 65 70 75 80 Pro Gln Phe Gly Gly Asn Pro Cys Glu Gly Pro Glu Lys Gln Thr Lys 85 90 95 Phe Cys Asn Ile Ala Leu Cys Pro Gly Arg Ala Val Asp Gly Asn Trp 100 105 110 Asn Glu Trp Ser Ser Trp Ser Ala Cys Ser Ala Ser Cys Ser Gln Gly 115 120 125 Arg Gln Gln Arg Thr Arg Glu Cys Asn Gly Pro Ser Tyr Gly Gly Ala 130 135 140 Glu Cys Gln Gly His Trp Val Glu Thr Arg Asp Cys Phe Leu Gln Gln 145 150 155 160 Cys Pro Val Asp Gly Lys Trp Gln Ala Trp Ala Ser Trp Gly Ser Cys 165 170 175 Ser Val Thr Cys Gly Ala Gly Ser Gln Arg Arg Glu Arg Val Cys Ser 180 185 190 Gly Pro 33 1336 PRT Homo sapiens 33 Thr Pro Ile Gly Arg Pro Arg Ile Arg His Gln Asp Lys Arg Thr Val 1 5 10 15 Asp Leu Thr Val Gln Val Pro Pro Ser Ile Ala Asp Glu Pro Thr Asp 20 25 30 Phe Leu Val Thr Lys His Ala Pro Ala Val Ile Thr Cys Thr Ala Ser 35 40 45 Gly Val Pro Phe Pro Ser Ile His Trp Thr Lys Asn Gly Ile Arg Leu 50 55 60 Leu Pro Arg Gly Asp Gly Tyr Arg Ile Leu Ser Ser Gly Ala Ile Glu 65 70 75 80 Ile Leu Ala Thr Gln Leu Asn His Ala Gly Arg Tyr Thr Cys Val Ala 85 90 95 Arg Asn Ala Ala Gly Ser Ala His Arg His Val Thr Leu His Val His 100 105 110 Glu Pro Pro Val Ile Gln Pro Gln Pro Ser Glu Leu His Val Ile Leu 115 120 125 Asn Asn Pro Ile Leu Leu Pro Cys Glu Ala Thr Gly Thr Pro Ser Pro 130 135 140 Phe Ile Thr Trp Gln Lys Glu Gly Ile Asn Val Asn Thr Ser Gly Arg 145 150 155 160 Asn His Ala Val Leu Pro Ser Gly Gly Leu Gln Ile Ser Arg Ala Val 165 170 175 Arg Glu Asp Ala Gly Thr Tyr Met Cys Val Ala Gln Asn Pro Ala Gly 180 185 190 Thr Ala Leu Gly Lys Ile Lys Leu Asn Val Gln Val Pro Pro Val Ile 195 200 205 Ser Pro His Leu Lys Glu Tyr Val Ile Ala Val Asp Lys Pro Ile Thr 210 215 220 Leu Ser Cys Glu Ala Asp Gly Leu Pro Pro Pro Asp Ile Thr Trp His 225 230 235 240 Lys Asp Gly Arg Ala Ile Val Glu Ser Ile Arg Gln Arg Val Leu Ser 245 250 255 Ser Gly Ser Leu Gln Ile Ala Phe Val Gln Pro Gly Asp Ala Gly His 260 265 270 Tyr Thr Cys Met Ala Ala Asn Val Ala Gly Ser Ser Ser Thr Ser Thr 275 280 285 Lys Leu Thr Val His Val Pro Pro Arg Ile Arg Ser Thr Lys Gly His 290 295 300 Tyr Thr Val Asn Glu Asn Ser Gln Ala Ile Leu Pro Cys Val Ala Asp 305 310 315 320 Gly Ile Pro Thr Pro Ala Ile Asn Trp Lys Lys Asp Asn Val Leu Leu 325 330 335 Ala Asn Leu Leu Gly Lys Tyr Thr Ala Glu Pro Tyr Gly Glu Leu Ile 340 345 350 Leu Glu Asn Val Val Leu Glu Asp Ser Gly Phe Tyr Thr Cys Val Ala 355 360 365 Asn Asn Ala Ala Gly Glu Asp Thr His Thr Val Ser Leu Thr Val His 370 375 380 Val Leu Pro Thr Phe Thr Glu Leu Pro Gly Asp Val Ser Leu Asn Lys 385 390 395 400 Gly Glu Gln Leu Arg Leu Ser Cys Lys Ala Thr Gly Ile Pro Leu Pro 405 410 415 Lys Leu Thr Trp Thr Phe Asn Asn Asn Ile Ile Pro Ala His Phe Asp 420 425 430 Ser Val Asn Gly His Ser Glu Leu Val Ile Glu Arg Val Ser Lys Glu 435 440 445 Asp Ser Gly Thr Tyr Val Cys Thr Ala Glu Asn Ser Val Gly Phe Val 450 455 460 Lys Ala Ile Gly Phe Val Tyr Val Lys Glu Pro Pro Val Phe Lys Gly 465 470 475 480 Asp Tyr Pro Ser Asn Trp Ile Glu Pro Leu Gly Gly Asn Ala Ile Leu 485 490 495 Asn Cys Glu Val Lys Gly Asp Pro Thr Pro Thr Ile Gln Trp Asn Arg 500 505 510 Lys Gly Val Asp Ile Glu Ile Ser His Arg Ile Arg Gln Leu Gly Asn 515 520 525 Gly Ser Leu Ala Ile Tyr Gly Thr Val Asn Glu Asp Ala Gly Asp Tyr 530 535 540 Thr Cys Val Ala Thr Asn Glu Ala Gly Val Val Glu Arg Ser Met Ser 545 550 555 560 Leu Thr Leu Arg Ser Pro Pro Ile Ile Thr Leu Glu Pro Val Glu Thr 565 570 575 Val Ile Asn Ala Gly Gly Lys Ile Ile Leu Asn Cys Gln Ala Thr Gly 580 585 590 Glu Pro Gln Pro Thr Ile Thr Trp Ser Arg Gln Gly His Ser Ile Ser 595 600 605 Trp Asp Asp Arg Val Asn Val Leu Ser Asn Asn Ser Leu Tyr Ile Ala 610 615 620 Asp Ala Gln Lys Glu Asp Thr Ser Glu Phe Glu Cys Val Ala Arg Asn 625 630 635 640 Leu Met Gly Ser Val Leu Val Arg Val Pro Val Ile Val Gln Val His 645 650 655 Gly Gly Phe Ser Gln Trp Ser Ala Trp Arg Ala Cys Ser Val Thr Cys 660 665 670 Gly Lys Gly Ile Gln Lys Arg Ser Arg Leu Cys Asn Gln Pro Leu Pro 675 680 685 Ala Asn Gly Gly Lys Pro Cys Gln Gly Ser Asp Leu Glu Met Arg Asn 690 695 700 Cys Gln Asn Lys Pro Cys Pro Val Asp Gly Ser Trp Ser Glu Trp Ser 705 710 715 720 Leu Trp Glu Glu Cys Thr Arg Ser Cys Gly Arg Gly Asn Gln Thr Arg 725 730 735 Thr Arg Thr Cys Asn Asn Pro Ser Val Gln His Gly Gly Arg Pro Cys 740 745 750 Glu Gly Asn Ala Val Glu Ile Ile Met Cys Asn Ile Arg Pro Cys Pro 755 760 765 Val His Gly Ala Trp Ser Ala Trp Gln Pro Trp Gly Thr Cys Ser Glu 770 775 780 Ser Cys Gly Lys Gly Thr Gln Thr Arg Ala Arg Leu Cys Asn Asn Pro 785 790 795 800 Pro Pro Ala Phe Gly Gly Ser Tyr Cys Asp Gly Ala Glu Thr Gln Met 805 810 815 Gln Val Cys Asn Glu Arg Asn Cys Pro Ile His Gly Lys Trp Ala Thr 820 825 830 Trp Ala Ser Trp Ser Ala Cys Ser Val Ser Cys Gly Gly Gly Ala Arg 835 840 845 Gln Arg Thr Arg Gly Cys Ser Asp Pro Val Pro Gln Tyr Gly Gly Arg 850 855 860 Lys Cys Glu Gly Ser Asp Val Gln Ser Asp Phe Cys Asn Ser Asp Pro 865 870 875 880 Cys Pro Thr His Gly Asn Trp Ser Pro Trp Ser Gly Trp Gly Thr Cys 885 890 895 Ser Arg Thr Cys Asn Gly Gly Gln Met Arg Arg Tyr Arg Thr Cys Asp 900 905 910 Asn Pro Pro Pro Ser Asn Gly Gly Arg Ala Cys Gly Gly Pro Asp Ser 915 920 925 Gln Ile Gln Arg Cys Asn Thr Asp Met Cys Pro Val Asp Gly Ser Trp 930 935 940 Gly Ser Trp His Ser Trp Ser Gln Cys Ser Ala Ser Cys Gly Gly Gly 945 950 955 960 Glu Lys Thr Arg Lys Arg Leu Cys Asp His Pro Val Pro Val Lys Gly 965 970 975 Gly Arg Pro Cys Pro Gly Asp Thr Thr Gln Val Thr Arg Cys Asn Val 980 985 990 Gln Ala Cys Pro Gly Gly Pro Gln Arg Ala Arg Gly Ser Val Ile Gly 995 1000 1005 Asn Ile Asn Asp Val Glu Phe Gly Ile Ala Phe Leu Asn Ala Thr 1010 1015 1020 Ile Thr Asp Ser Pro Asn Ser Asp Thr Arg Ile Ile Arg Ala Lys 1025 1030 1035 Ile Thr Asn Val Pro Arg Ser Leu Gly Ser Ala Met Arg Lys Ile 1040 1045 1050 Val Ser Ile Leu Asn Pro Ile Tyr Trp Thr Thr Ala Lys Glu Ile 1055 1060 1065 Gly Glu Ala Val Asn Gly Phe Thr Leu Thr Asn Ala Val Phe Lys 1070 1075 1080 Arg Glu Thr Gln Val Glu Phe Ala Thr Gly Glu Ile Leu Gln Met 1085 1090 1095 Ser His Ile Ala Arg Gly Leu Asp Ser Asp Gly Ser Leu Leu Leu 1100 1105 1110 Asp Ile Val Val Ser Gly Tyr Val Leu Gln Leu Gln Ser Pro Ala 1115 1120 1125 Glu Val Thr Val Lys Asp Tyr Thr Glu Asp Tyr Ile Gln Thr Gly 1130 1135 1140 Pro Gly Gln Leu Tyr Ala Tyr Ser Thr Arg Leu Phe Thr Ile Asp 1145 1150 1155 Gly Ile Ser Ile Pro Tyr Thr Trp Asn His Thr Val Phe Tyr Asp 1160 1165 1170 Gln Ala Gln Gly Arg Met Pro Phe Leu Val Glu Thr Leu His Ala 1175 1180 1185 Ser Ser Val Glu Ser Asp Tyr Asn Gln Ile Glu Glu Thr Leu Gly 1190 1195 1200 Phe Lys Ile His Ala Ser Ile Ser Lys Gly Asp Arg Ser Asn Gln 1205 1210 1215 Cys Pro Ser Gly Phe Thr Leu Asp Ser Val Gly Pro Phe Cys Ala 1220 1225 1230 Asp Glu Asp Glu Cys Ala Ala Gly Asn Pro Cys Ser His Ser Cys 1235 1240 1245 His Asn Ala Met Gly Thr Tyr Tyr Cys Ser Cys Pro Lys Gly Leu 1250 1255 1260 Thr Ile Ala Ala Asp Gly Arg Thr Cys Gln Asp Ile Asp Glu Cys 1265 1270 1275 Ala Leu Gly Arg His Thr Cys His Ala Gly Gln Asp Cys Asp Asn 1280 1285 1290 Thr Ile Gly Ser Tyr Arg Cys Val Val Arg Cys Gly Ser Gly Phe 1295 1300 1305 Arg Arg Thr Ser Asp Gly Leu Ser Cys Gln Asp Ile Asn Glu Cys 1310 1315 1320 Gln Glu Ser Ser Pro Val Thr Ser Ala Val Ser Met Pro 1325 1330 1335 34 4073 DNA Homo sapiens 34 actcctatag ggcggccgcg aattcggcac caggataaaa gaactgtgga tctcactgtc 60 caagttccac cttccatagc tgatgagcct acagatttcc tagtaaccaa acatgcccca 120 gcagtaatta cctgcactgc ttcgggagtt ccatttccct caattcactg gaccaaaaat 180 ggtataagac tgcttcccag gggagatggc tatagaattc tgtcctcagg agcaattgaa 240 atacttgcca cccaattaaa ccatgctgga agatacactt gtgtcgctag gaatgcggct 300 ggctctgcac atcgacacgt gacccttcat gttcatgagc ctccagtcat tcagccccaa 360 ccaagtgaac tacacgtcat tctgaacaat cctattttat taccatgtga agcaacaggg 420 acacccagtc ctttcattac ttggcaaaaa gaaggcatca atgttaacac ttcaggcaga 480 aaccatgcag ttcttcctag tggcggctta cagatctcca gagctgtccg agaggatgct 540 ggcacttaca tgtgtgtggc ccagaacccg gctggtacag ccttgggcaa aatcaagtta 600 aatgtccaag ttcctccagt cattagccct catctaaagg aatatgttat tgctgtggac 660 aagcccatca cgttatcctg tgaagcagat ggcctccctc cgcctgacat tacatggcat 720 aaagatgggc gtgcaattgt ggaatctatc cgccagcgcg tcctcagctc tggctctctg 780 caaatagcat ttgtccagcc tggtgatgct ggccattaca cgtgcatggc agccaatgta 840 gcaggatcaa gcagcacaag caccaagctc accgtccatg taccacccag gatcagaagt 900 acaaaaggac actacacggt caatgagaat tcacaagcca ttcttccatg cgtagctgat 960 ggaatcccca caccagcaat taactggaaa aaagacaatg ttcttttagc taacttgtta 1020 ggaaaataca ctgctgaacc atatggagaa ctcattttag aaaatgttgt gctggaggat 1080 tctggcttct atacctgtgt tgctaacaat gctgcaggtg aagatacaca cactgtcagc 1140 ctgactgtgc atgttctccc cacttttact gaacttcctg gagacgtgtc attaaataaa 1200 ggagaacagc tacgattaag ctgtaaagct actggtattc cattgcccaa attaacatgg 1260 accttcaata acaatattat tccagcccac tttgacagtg tgaatggaca cagtgaactt 1320 gttattgaaa gagtgtcaaa agaggattca ggtacttatg tgtgcaccgc agagaacagc 1380 gttggctttg tgaaggcaat tggatttgtt tatgtgaaag aacctccagt cttcaaaggt 1440 gattatcctt ctaactggat tgaaccactt ggtgggaatg caatcctgaa ttgtgaggtg 1500 aaaggagacc ccaccccaac catccagtgg aacagaaagg gagtggatat tgaaattagc 1560 cacagaatcc ggcaactggg caatggctcc ctggccatct atggcactgt taatgaagat 1620 gccggtgact atacatgtgt agctaccaat gaagctgggg tggtggagcg cagcatgagt 1680 ctgactctgc gaagtcctcc tattatcact cttgagccag tggaaactgt tattaatgct 1740 ggtggcaaaa tcatattgaa ttgtcaggca actggagagc ctcaaccaac cattacatgg 1800 tcccgtcaag ggcactctat ttcctgggat gaccgggtta acgtgttgtc caacaactca 1860 ttatatattg ctgatgctca gaaagaagat acctctgaat ttgaatgtgt tgctcgaaac 1920 ttaatgggtt ctgtccttgt cagagtgcca gtcatagtcc aggttcatgg tggattttcc 1980 cagtggtctg catggagagc ctgcagtgtc acctgtggaa aaggcatcca aaagaggagt 2040 cgtctgtgca accagcccct tccagccaat ggtgggaagc cctgccaagg ttcagatttg 2100 gaaatgcgaa actgtcaaaa taagccttgt ccagtggatg gtagctggtc ggaatggagt 2160 ctttgggaag aatgcacaag gagctgtgga cgcggcaacc aaaccaggac caggacttgc 2220 aataatccat cagttcagca tggtgggcgg ccatgtgaag ggaatgctgt ggaaataatt 2280 atgtgcaaca ttaggccttg cccagttcat ggagcatgga gcgcttggca gccttgggga 2340 acatgcagcg aaagttgtgg gaaaggtact cagacaagag caagactttg taataaccca 2400 ccaccagcgt ttggtgggtc ctactgtgat ggagcagaaa cacagatgca agtttgcaat 2460 gaaagaaatt gtccaattca tggcaagtgg gcgacttggg ccagttggag tgcctgttct 2520 gtgtcatgtg gaggaggtgc cagacagaga acaaggggct gctccgaccc tgtgccccag 2580 tatggaggaa ggaaatgcga agggagtgat gtccagagtg atttttgcaa cagtgaccct 2640 tgcccaaccc atggtaactg gagtccttgg agtggctggg gaacatgcag ccggacgtgt 2700 aacggagggc agatgcggcg gtaccgcaca tgtgataacc ctcctccctc caatggggga 2760 agagcttgtg ggggaccaga ctcccagatc cagaggtgca acactgacat gtgtcctgtg 2820 gatggaagtt ggggaagctg gcatagttgg agccagtgct ctgcctcctg tggaggaggt 2880 gaaaagactc ggaagcggct gtgcgaccat cctgtgccag ttaaaggtgg ccgtccctgt 2940 cccggagaca ctactcaggt gaccaggtgc aatgtacaag catgtccagg tgggccccag 3000 cgagccagag gaagtgttat tggaaatatt aatgatgttg aatttggaat tgctttcctt 3060 aatgccacaa taactgatag ccctaactct gatactagaa taatacgtgc caaaattacc 3120 aatgtacctc gtagtcttgg ttcagcaatg agaaagatag tttctattct aaatcccatt 3180 tattggacaa cagcaaagga aataggagaa gcagtcaatg gctttaccct caccaatgca 3240 gtcttcaaaa gagaaactca agtggaattt gcaactggag aaatcttgca gatgagtcat 3300 attgcccggg gcttggattc cgatggttct ttgctgctag atatcgttgt gagtggctat 3360 gtcctacagc ttcagtcacc tgctgaagtc actgtaaagg attacacaga ggactacatt 3420 caaacaggtc ctgggcagct gtacgcctac tcaacccggc tgttcaccat tgatggcatc 3480 agcatcccat acacatggaa ccacaccgtt ttctatgatc aggcacaggg aagaatgcct 3540 ttcttggttg aaacacttca tgcatcctct gtggaatctg actataacca gatagaagag 3600 acactgggtt ttaaaattca tgcttcaata tccaaaggag atcgcagtaa tcagtgcccc 3660 tccgggttta ccttagactc agttggacct ttttgtgctg atgaggatga atgtgcagca 3720 gggaatccct gctcccatag ctgccacaat gccatgggga cttactactg ctcctgccct 3780 aaaggcctca ccatagctgc agatggaaga acttgtcaag atattgatga gtgtgctttg 3840 ggtaggcata cctgccacgc tggtcaggac tgtgacaata cgattggatc ttatcgctgt 3900 gtggtccgtt gtggaagtgg ctttcgaaga acctctgatg ggctgagttg tcaagatatt 3960 aatgaatgtc aagaatccag ccctgtcacc agcgctgttt caatgccata ggaagtttcc 4020 attgtggatg tgaacctggg tatcagctca aaggcagaaa atgcatggat tgt 4073 

What is claimed is:
 1. A method of modulating angiogenesis at a site, the method comprising causing an effective amount of a composition comprising a BTL.012-like protein to be supplied to the site.
 2. The method of claim 1 wherein the BTL.012-like protein has an amino acid sequence identical to SEQ ID NO:1.
 3. The method of claim 1 wherein the BTL.012-like protein has an amino acid sequence which is at least 60% identical over at least 40 residues to SEQ ID NO:1.
 4. The method of claim 1 wherein the BTL.0 12-like protein has an amino acid sequence which is at least 70% identical over at least 30 residues to SEQ ID NO:1.
 5. A method of modulating the formation of cells into capillary-like structures comprising contacting the cells with a biologically effective amount of a composition comprising a BTL.012-like protein.
 6. The method of claim 5 wherein the cells are endothelial cells of human origin.
 7. A protein characterized by having a deduced amino acid sequence which is at least 60% identical over 40 residues to SEQ ID NO:1.
 8. The protein according to claim 7, wherein the deduced amino acid sequence is at least 80% identical over 50 residues to SEQ ID NO:1.
 9. A pharmaceutical composition for modulating angiogenesis comprising a protein characterized by having a deduced amino acid sequence which is at least 60% identical over 40 residues to SEQ ID NO:1 and a pharmaceutically acceptable carrier.
 10. The method of claim 1, wherein the site is within a human patient and the protein is supplied to the site via a pharmaceutical composition according to claim
 9. 11. The method of claim 10, wherein the site is within a human patient and the protein is supplied to the site via a process of gene therapy.
 12. A method for preventing, treating, or ameliorating a medical condition in an individual, the method comprising providing a source of an effective amount of at least one protein according to claim 7 to the individual.
 13. The method of claim 12, wherein the protein is supplied to the individual by providing to the individual a source of a polynucleotide encoding the protein and expressing the protein in vivo.
 14. The method of claim 12, wherein the medical condition is selected from the group consisting of cancer, metastasis, diabetic retinopathy, macular degeneration, cardiovascular disease, and a wound.
 15. A polynucleotide selected from the group consisting of (a) a polynucleotide coding for a protein according to claim 7; (b) a polynucleotide complementary to (a); (c) a polynucleotide having at least 90% identity over at least 20 bases to SEQ ID NO:34; and (d) a polynucleotide complementary to (c).
 16. The polynucleotide according to claim 15, wherein the polynucleotide is operably linked within an expression vector to a promoter, the expression vector thus being capable of being used to express the protein according to claim
 1. 17. A method for producing a protein according to claim 7 comprising the steps of (a) introducing an expression vector capable of expressing the protein according to claim 7 into a cell capable of expressing the protein according to claim 7, (b) growing cells resulting from step (a) under conditions sufficient to allow the cells to express the protein according to claim 7, and (c) recovering the protein according to claim 7 from the result of step (b).
 18. An antibody against a protein according to claim
 7. 19. A method for diagnosing a disease or medical condition or susceptibility to a disease or medical condition, the disease or medical condition related to inadequate or excess expression of a protein according to claim 7, the method comprising the steps (a) determining the level of expression of said protein in a sample; and (b) comparing the level of expression of said protein against a standard to make a diagnosis.
 20. The method of claim 19, wherein the medical condition is selected from the group consisting of cancer, metastasis, diabetic retinopathy, macular degeneration, cardiovascular disease, and a wound.
 21. A protein characterized by having a deduced amino acid sequence which is at least 60% identical over 40 residues to SEQ ID NO:33. 